[Changes in the antigenicity of influenza virus neuraminidases due to various methods of preparation].
Identifieur interne : 002961 ( Main/Exploration ); précédent : 002960; suivant : 002962[Changes in the antigenicity of influenza virus neuraminidases due to various methods of preparation].
Auteurs : U. DesselbergerSource :
- Fortschritte der Medizin [ 0015-8178 ] ; 1978.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Orthomyxoviridae.
- immunologie : Sialidase.
- isolement et purification : Hémagglutinines, Sialidase.
- Anticorps antiviraux, Antigènes viraux, Méthodes, Recombinaison génétique, Réactions croisées.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Neuraminidase.
- chemical , isolation & purification : Hemagglutinins, Neuraminidase.
- chemical : Antibodies, Viral, Antigens, Viral.
- enzymology : Orthomyxoviridae.
- Cross Reactions, Methods, Recombination, Genetic.
Abstract
The influenza virus strains A/Sing/1/57 (H2N2), A/Bel/42 (HoN1) and A/Bel/42 (Ho)-A/Sing/1/57 (N2) were treated with bromelain under reducing conditions and with reducing agent alone, and the antigenicity of the neuraminidase (NA) of intact virus and of the split products was tested comparatively. It was found that the antigenicity of NA was influenced quantitatively and qualitatively by the preparation procedure. Antineuraminidase (AN) antibodies obtained after vaccination of guinea pigs with intact virus and with split products differed in their cross-reactivity with heterologous neuraminidases. AN antibodies directed against N2 NA on the recombinant differed qualitatively from those directed against N2 NA of parent virus. The results warrant the conclusion that the antigenicity of isolated NA or of NA on recombinant virus can differ from that of the NA on intact homologous virus and that such alterations could influence the determination of antigenic relationship between neuraminidases.
PubMed: 627393
Affiliations:
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Le document en format XML
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Desselberger</name></author></analytic><series><title level="j">Fortschritte der Medizin</title><idno type="ISSN">0015-8178</idno><imprint><date when="1978" type="published">1978</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term><term>Cross Reactions</term><term>Hemagglutinins (isolation & purification)</term><term>Methods</term><term>Neuraminidase (immunology)</term><term>Neuraminidase (isolation & purification)</term><term>Orthomyxoviridae (enzymology)</term><term>Recombination, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux</term><term>Antigènes viraux</term><term>Hémagglutinines (isolement et purification)</term><term>Méthodes</term><term>Orthomyxoviridae (enzymologie)</term><term>Recombinaison génétique</term><term>Réactions croisées</term><term>Sialidase (immunologie)</term><term>Sialidase (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Neuraminidase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Hemagglutinins</term><term>Neuraminidase</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Sialidase</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Hémagglutinines</term><term>Sialidase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cross Reactions</term><term>Methods</term><term>Recombination, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anticorps antiviraux</term><term>Antigènes viraux</term><term>Méthodes</term><term>Recombinaison génétique</term><term>Réactions croisées</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The influenza virus strains A/Sing/1/57 (H2N2), A/Bel/42 (HoN1) and A/Bel/42 (Ho)-A/Sing/1/57 (N2) were treated with bromelain under reducing conditions and with reducing agent alone, and the antigenicity of the neuraminidase (NA) of intact virus and of the split products was tested comparatively. It was found that the antigenicity of NA was influenced quantitatively and qualitatively by the preparation procedure. Antineuraminidase (AN) antibodies obtained after vaccination of guinea pigs with intact virus and with split products differed in their cross-reactivity with heterologous neuraminidases. AN antibodies directed against N2 NA on the recombinant differed qualitatively from those directed against N2 NA of parent virus. The results warrant the conclusion that the antigenicity of isolated NA or of NA on recombinant virus can differ from that of the NA on intact homologous virus and that such alterations could influence the determination of antigenic relationship between neuraminidases.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Desselberger, U" sort="Desselberger, U" uniqKey="Desselberger U" first="U" last="Desselberger">U. Desselberger</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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